A - Full Service Sequencing (includes sequencing reaction***, cleanup, and run on the 3130xl)

B - Cleanup and Run Sequencing Reactions on the 3130xl (reaction performed by user)

C - Run Sequencing Reactions on the 3130xl (user performs reactions and cleanup)

D - Run Fragment Analysis Samples on the 3130xl (User performs reaction and provides size standard)

E - RNA Nano 6000 Analysis on Agilent 2100 BioAnalyzer****

* All samples that are not submitted in multiples of 16 will be charged the single sample price (e.g., if 19 samples are submitted, 16 will be charged at the bulk rate and 3 will be charged at the single sample rate). Such samples may experience a processing delay. If expedited processing is required, an additional fee of $15 can be added.

** We ask that you leave one well open for us to run a positive control.

*** Assumes M13 or other standard primers. Custom primers should be provided by individual researchers. Submission conditions vary depending on sample type. Please contact the lab manager for details or look here.

****Please provide at least one week of advance notice for use of BioAnalyzer Chips to allow time for ordering, if necessary.

Working Hours: 10:00 AM to 4:30 PM (Monday to Friday)

Day of Sequencing: Tuesday and Thursday

Sample Drop-Off: By appointment. Please contact Alex Harris (wvugenomics@gmail.com) or call 304 293-5201 ext 31456 or ext 31512. Make an appointment with Alex Harris (wvugenomics@gmail.com) or Stephen DiFazio (stephen.difazio@mail.wvu.edu) to drop off samples. We must receive samples by Monday or Wednesday for Tuesday and Thursday sequencing, respectively.

Template requirement: PCR product (Purified): 10 μL of at least 30 ng /μL, or provide gel image of purified PCR products. For plasmid DNA: 10 μL of 200-300-ng/μL or gel image. Please mention "μL" of DNA or plasmid loaded in each well. Primer Requirement: 10.0 μL of 2.5 pmol/μL related primer.

Primers in stock:

• M13F (5' GTA AAA CGA GGG CCA G 3')
• M13R (5' GAG GAA ACA GCT ATG AC 3')
• T3 (5' ATT AAC CCT CAC TAA AG 3')
• T7 (5' TAA TAC GAC TCA CTA TAG GG 3')

NOTE:

• Both DNA and primer should be pure and free of EDTA, Protein, RNA, Ethanol and Salt. Please DON'T suspend Primer in TE buffer. We prefer elution in sterile de-ionized water, instead of EB buffer, if you are using a Qiagen MiniPrep kit for purification.
• Primers should be in the range of 18-25 bp with 40-60% GC content.
• Please fill in the following table and submit along with your samples. Please include a separate sheet for each plate. Leave plate row and col blank if samples are in tubes. Number tubes according to the first column.

Full Service Gene Chip Hybridization

Includes cDNA synthesis, labeling, hybridization, scanning, and normalization.

Gene Chip Scanning, Hybridization, and Normalization

RNA Extraction

E - RNA Nano 6000 Analysis on Agilent 2100 BioAnalyzer***