On-going research in the area of affinity interaction includes the study of proteins and peptides implicated in disease. Dynamic affinity capillary electrophoresis as well as immobilized selection, offer a means of rapidly estimating binding constants or rapidly identifying binding agents. This research focuses on the development and refinement of enabling technology; providing separation based assays to quantify affinity and to directly select for affinity agents. We are particularly interested in characterizing the interaction between heparin-like glycosaminoglycans and protein binding domains using capillary electrophoresis [1]. This work ties into physiological signaling, and ultimately research in disease and dysfunction.

1. J McKeon, LA Holland*, “The Determination of Dissociation Constants for a Heparin-Binding Domain of Amyloid Precursor Protein and Heparins or Heparan Sulfate by Affinity Capillary Electrophoresis”, Electrophoresis 25(9), pp. 1243-1248, 2004.