BIOL 219 – The Living Cell:

Chapter 10 - Sample test questions. Chick on the answer you think is most appropriate to see if you are right.

What will be the result of omitting the dNTPs (dATP, dGTP, dCTP, and dTTP) in a PCR?
A. The reaction will proceed normally.
B. The primers will not anneal to the template strand properly.
C. The temperature of the reaction will never get high enough to induce denaturation.
D. Strand extension will not occur.
E. Taq polymerase will denature and not function.

Which of the following steps is NOT required for the construction of a DNA library?
A. Fragmentation of genomic DNA with restriction enzymes.
B. Sequencing of the fragments.
C. Insertion of the fragments into a vector such as a plasmid.
D. Insertion of the fragment-vector combination into cells.
E. All of the above steps are required.

You have a pool of DNA fragments in a tube. You nothing about their sequences. Which of the following techniques is most likely to allow you to determine the size range of the fragments?
A. PCR
B. DNA hybridization
C. Gel electrophoresis
D. DNA cloning
E. None of the above would help

A farmer breeding only the sheep that consistently produce the most wool is an example of:
A. artificial selection.
B. DNA cloning.
C. DNA hybridization.
D. a genomic library.
E. a germ-line mutation.

In order to create a genetically altered strain of mice that faithfully display the introduced mutation generation after generation:
A. you must introduce the mutation into the germ line.
B. you must introduce the mutation into embryonic stem cells.
C. embryonic stem cells containing the mutation must be introduced into a mouse embryo.
D. all of the above.
E. only B and C.